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Becton Dickinson aec substrate buffer
Aec Substrate Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/substrate+buffer+aec/aec+substrate+buffer/pmc08675757-398-9-12
Average 90 stars, based on 1 article reviews
aec substrate buffer - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Incubation:

Article Title: Cross-Protection against MERS-CoV by Prime-Boost Vaccination Using Viral Spike DNA and Protein
Article Snippet: .. After discarding the cells and 195 peptide stimulants and washing, biotinylated anti-mouse IFN-γ (BD Bioscience) was added to 196 each well, and the plate was incubated at RT for 2 h. After washing the wells, streptavidin-197 HRP (BD Bioscience) was added, and the plate was incubated at RT for 1 h. After washing, 198 AEC Substrate Solution (BD TM AEC Substrate Reagent Set; BD Biosciences) was added to 199 each well. .. Spot development was stopped by washing the wells with deionized water, and 200 spots were counted by using an ELISpot reader (Cellular Technology Limited, Cleveland, OH, 201 USA).

Article Title: Plant-Derived Extracellular Vesicles as a Delivery Platform for RNA-Based Vaccine: Feasibility Study of an Oral and Intranasal SARS-CoV-2 Vaccine
Article Snippet: Then, plates were washed three times with washing buffer and incubated with streptavidin–HRP (557630, BD Bioscience, Eysins, Switzerland) for 1 h at room temperature. .. Following that, plates were washed four times with washing buffer and twice with PBS, then incubated with substrate solution (AEC substrate set, BD Bioscience, Eysins, Switzerland) for 15 min until spot development. ..

Blocking Assay:

Article Title: The B Cell Activation-Induced miR-183 Cluster Plays a Minimal Role in Canonical Primary Humoral Responses
Article Snippet: The PCR products were gel-purified and cloned into Zero Blunt TOPO (no. K280020; Invitrogen). .. PBS plus 2% BSA was used as blocking buffer, ELISpot AEC substrate (no. 551951; BD Biosciences) was used to develop. .. Sequence analysis was performed using SeqMan Pro (DNASTAR).

Enzyme-linked Immunospot:

Article Title: The B Cell Activation-Induced miR-183 Cluster Plays a Minimal Role in Canonical Primary Humoral Responses
Article Snippet: The PCR products were gel-purified and cloned into Zero Blunt TOPO (no. K280020; Invitrogen). .. PBS plus 2% BSA was used as blocking buffer, ELISpot AEC substrate (no. 551951; BD Biosciences) was used to develop. .. Sequence analysis was performed using SeqMan Pro (DNASTAR).

other:

Article Title: Marek’s disease virus-specific T cells proliferate, express antiviral cytokines but have impaired degranulation response
Article Snippet: The assay was developed in the presence of 3-Amino-9-ethylcarbazole (AEC) substrate solution (BD Biosciences, UK).

Article Title: A Multistage Antigen Complex Epera013 Promotes Efficient and Comprehensive Immune Responses in BALB/c Mice
Article Snippet: After 24 h of incubation in 37 °C, 5% CO 2 and humidified incubator, the plates were washed and added with biotin-conjugated detection antibody and incubated at room temperature for 2 h. After the plates were washed and added with streptavidin labeled HRP and incubated at room temperature for 1 h and washed again, 100 μL 3-amino-9-ethylcarbazole (AEC) substrate solution (BD Biosciences, Franklin Lakes, NJ, USA) was added and incubated at room temperature for distinct spot formation, finally the reaction was terminated by deionized water rinse.



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